Korean J Bone Metab > Volume 19(1); 2012 > Article
Korean Journal of Bone Metabolism 2012;19(1):35-46.
The Effects of Extracellular pH on Proliferation and Differentiation of human Bone Marrow Stem Cells
세포 외 pH 변화가 인간 골수 기질세포의 증식 및 분화에 미치는 영향
임예현1,남태석2,김정화1,이강식1,이동호1,윤준오1,장재석1*
울산대학교 의과대학1, 서울나누리병원2
Abstract
Objective
s: The purpose of this study is to identify whether the change of pH affects the proliferation and the differentiation of human bone marrow stem cells (hBMSCs) and what mechanism is underlied.
Methods
To achieve objective of this study, hBMSCs were cultivated in the conditioned media adjusted to potential of hydrogen (pH) ranging from 6.4 to 8.0 using addition of hydrochloric acid (HCl) and sodium hydroxide (NaOH). The ratio of proliferation of hBMSCs according to the change of pH was measured for 24 h, 48 h, and 72 h using water-soluble tetrazolium salt (WST)-8 method. To elucidate the mechanism involved, hBMSCs was subjected to blocking extracellular signal-regulated kinases (ERK) and calcium sensing receptor (CaSR) activation. The Osteogenicrelated genes and alkaline phosphatase (ALP) activity were tested under the conditioned media.
Results
The proliferation of hBMSCs was promoted under extracellular alkali conditions (pH 7.6~8.0) via CaSR/ ERK pathway. On the other hand, the differentiation was inhibited/delayed via decreased ALP activity besides gene expression at pH 8.0.
Conclusion
Extracellular alkali or acidic surrounding according to pH alteration can play a crucial role in hBMSC behavior including the proliferation and the differentiation.
Key Words: Bone marrow cells, Cell differentiation, Cell proliferation, pH, Stem cells


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