The Effects of Extracellular pH on Proliferation and Differentiation of
human Bone Marrow Stem Cells |
세포 외 pH 변화가 인간 골수 기질세포의 증식 및
분화에 미치는 영향 |
임예현1,남태석2,김정화1,이강식1,이동호1,윤준오1,장재석1* |
울산대학교 의과대학1, 서울나누리병원2 |
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Abstract |
Objective s: The purpose of this study is to identify whether the change of pH affects the proliferation and the
differentiation of human bone marrow stem cells (hBMSCs) and what mechanism is underlied.
Methods To achieve objective of this study, hBMSCs were cultivated in the conditioned media adjusted to potential
of hydrogen (pH) ranging from 6.4 to 8.0 using addition of hydrochloric acid (HCl) and sodium hydroxide (NaOH).
The ratio of proliferation of hBMSCs according to the change of pH was measured for 24 h, 48 h, and 72 h using
water-soluble tetrazolium salt (WST)-8 method. To elucidate the mechanism involved, hBMSCs was subjected to
blocking extracellular signal-regulated kinases (ERK) and calcium sensing receptor (CaSR) activation. The Osteogenicrelated
genes and alkaline phosphatase (ALP) activity were tested under the conditioned media.
Results The proliferation of hBMSCs was promoted under extracellular alkali conditions (pH 7.6~8.0) via CaSR/
ERK pathway. On the other hand, the differentiation was inhibited/delayed via decreased ALP activity besides gene
expression at pH 8.0.
Conclusion Extracellular alkali or acidic surrounding according to pH alteration can play a crucial role in hBMSC
behavior including the proliferation and the differentiation. |
Key Words:
Bone marrow cells, Cell differentiation, Cell proliferation, pH, Stem cells |
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