| Effect of Rehmannia glutinosa Libosch and Acanthopanax senticosus Extracts
on Osteoblastic activity and Proliferation and Osteoclastic Bone Resorption
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| 숙지황과 가시오가피 추출물이 조골세포의 활성과 증식 및
파골세포의 골흡수 억제에 미치는 효과
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| 김정근1,김세원2,김종여3,고선일1,김현만4,백정화5,류현모6,오귀옥7 |
| 1단국대학교 치과대학 구강생화학교실, 2단국대학교 치과대학 치과약리학교실,
3단국대학교 치과대학 구강조직학교실, 4서울대학교 치과대학 구강해부학교실,
5서울대학교 치과대학 치과약리학교실, 6경북대학교 치과대학 구강생화학교실,
7주식회사 오스코텍
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| Abstract |
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The present study was performed to investigate whether two herbal extracts play roles in the bone metabolism. We examined cellular activities of osteoblasts by measurement of cell proliferation rate, alkaline phosphatase (ALP) activity, and transcription promoting activity of Runx2. Osteoclast activity was assayed by measuring the area of resorption pits after culture of osteoclast precursor cells on the calcium-phosphate coated culture dish (OAASTM, OCT Inc.). There was an maximum 130% increase in proliferation rate of osteoblastic cells (MG63) after treatment with fraction Rehmannia glutinosa, and 120% increase with fraction Acanthopanax, when compared to the control. Rehmannia glutinosa-treated osteoblastic cells (HOS) showed a statistically significant increase in ALP activity. Also both of fractions increased the Runx2-induced luciferase activity, which was detected by p6xOSE2-Luc-transient transfected C2C12 cells. Rehmannia glutinosa and Acanthopanax showed significant decreases in the area of resorption pits. Taken together, Rehmannia glutinosa and Acanthopanax stimulate the proliferation and bioactivities of bone-forming osteoblasts, and inhibit activities of bone-resorbing osteoclasts. We believe that these fractions contain effective substances that have the potential to overcome osteoporosis. |
| Key Words:
Rehmannia glutinosa, Acanthopanax, Osteoporosis, Osteoclast, Osteoblast |
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