Association between Bone Mineral Density and Interleukin-6 Gene
Promoter Polymorphism in Healthy Young Korean Men
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한국인 젊은 남성에서 골밀도와 IL-6 유전자
다형성과의 상관관계
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김은숙,고정민1,김영일,홍정수2,김신윤3,김기수1 |
울산대학교 의과대학 울산대학병원 내과, 서울아산병원 내과1, 아산생명과학연구소2,
경북대학교 의과대학 정형외과3
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Abstract |
Background The peak bone mass is recognized as an important determinant in the development of osteoporosis and genetic factors are the deciding determinants of peak bone mass. The cytokine interleukin-6 (IL-6) has important effects on osteoclast differentiation and function and it has been implicated in the pathogenesis of bone loss associated with estrogen deficiency. Recently, a G/C polymorphism in position -174 of the IL-6 gene promoter was found. We investigated associations between bone mineral density (BMD) and polymorphisms of the IL-6 gene in young men.
Methods From 225 healthy volunteers aged 20~39 years, we identified the IL-6 genotypes (G/G, G/C, and C/C) using the polymerase chain reaction (PCR)-restriction fragment length polymorphism method. The PCR products were digested with NlaIII. BMD at the lumbar spine and femoral neck was measured using dual energy X-ray absorptiometry.
Results & Conclusion: All subjects were G/G homozygous variants and no C allelic variants were found. The results suggest that the -174 G/C polymorphism of IL-6 gene may be absent or very rare in Korean men and IL-6 polymorphism is unlikely to be a important determinant of peak bone mass in Korean men.
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Key Words:
Interleukin-6 gene, Polymorphism, Bone density, Men |
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