| Effect of the Long-term Administration of Pamidronate on Bone Strength
and Fracture Healing in a Rat Model
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| 백서모델에서 장기간 파미드로네이트 투여가 골 강도와
골절 치유에 미치는 영향
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| 양규현,박희완,원정훈,유제현,류종현1,추교석1,김재신1 |
| 연세대학교 의과대학 영동세브란스병원 정형외과, 한림제약 중앙연구소1 |
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| Abstract |
Background
Increased bone mineral density and reduced fracture incidences have been achieved by using bisphosphonates in osteoporosis and osteogenesis imperfecta. However, they cannot eliminate fracture episodes completely. Therefore, it is important to determine whether the long-term administration of strong antiresorptive agents compromises bone strength and the fracture healing procedure. So, We investigated the effects of the long-term administration of pamidronate on long bone strength and fracture healing in rat osteoporosis model.
Methods
We performed bilateral ovariectomy in sixty-six Sprague-Dawley female rats, average age is 3-month-old. Three months after ovariectomy, animals were randomized into two groups (n=33). Disodium pamidronate (0.5 mg/kg) was injected every month for 6 months in the treated group, and physiologic saline was administered in the control group. After the six-month administarion periods, the left femoral shaft was fractured by the Bonnarens and Einhorn technique after intramedullary nailing with 1.14 mm nonthreaded K-wire. Five weeks after fracture, rats were euthanized and both femurs were removed. We checked the mechanical properties of normal (right) and fractured (left) femurs and the bone mineral density of the right femoral head. Pamidronate concentration in bone was checked by high-performance liquid chromatography.
Results
The maximum strength of right femora was 154.4±17.5 N in the pamidronate-treated group and 129.7±28.0 N in the control group (p=0.0002), and stiffness was 452.5±122.8 N/mm in the pamidronate-treated group and 378.6±126.2 N/mm in the control group (p=0.036). Maximum strength of the callus was 22.7±7.2% compared to right femur in the pamidronate-treated group and 29.7±13.5% in the control group (p=0.025). The mean bone mineral density of the right femoral head was 230±9 mg/cm2 in pamidronate-treated group and 219±13 mg/cm2 in the control group (p=0.0007). The mean pamidronate concentration in bon |
| Key Words:
Pamidronate, Osteoporosis, Fracture healing |
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